Mesenchymal Stem Cell Therapy in Local Radiation Injuries-A Japanese Approach

Sadanori Akita, Hiroshi Yoshimoto, Kozo Akino, Akira Ohtsuru, Kenji Hayashida, Akiyoshi Hirano, Keishi Suzuki, Shunichi Yamashita
Department of Plastic and Reconstructive Surgery, Nagasaki University Hospital
Contact e-mail: akitas@hf.rim.or.jp

Abstract

Local radiation injury takes sometimes very unfavorable clinical course such as intractable and prolonged wounds. There are clinical evidences that the local somatic stem cells are expressed in the adjacent to of the injured wounds. Among such somatic stem cells, adipose-derived regenerative cells (ADRCs) are highly expected cell source, because the donor-site morbidity is minimal and quickness and easiness of cell processing in a closed circuit. As our consecutive 10 clinical series, aged 47 to 89 years (62.5 ± 14.2 years) with more than 30 years of duration after sequential 50 Gy of the radiation-induced wound treatment with ADRCs redeemed the regenerative wound healing and local tissue regeneration including tendon, bone, fat and muscle as well as skin re-surfacing. All cases regenerated and healed within 10 weeks and demonstrate no recurrence over 2 years post-operatively. In vitro cell culture derived from the patients' own ADRCs demonstrated proliferative and can be differentiated to the adipocytes by BODIPY live analysis as well as by induction with IBMX, insulin, indomethacin and dexamethasone and confirmed by oil red O staining.

We therefore, promote this easy and safe method for intractable radiation-induced local injuries more cases and conclude this would be the first choice of therapy for such cases.