Recommended Approach for Multi-Parameter Biodosimetry

William F. Blakely PhD and Natalia I. Ossetrova PhD
Armed Forces Radiobiology Research Institute, Bethesda, Maryland USA

Abstract

The need to rapidly access radiation injury in mass casualty and population monitoring scenarios has prompted an evaluation of: a) suitable biomarkers that can provide early diagnostic information after radiation exposure and b) prioritization process.  We advocate use of a multiple parameter bioassay approach based on novel molecular, hematological, and cytological biomarkers that are rapid and scalable for high-throughput analysis.  These biomarkers need to be validated in suitable radiation model systems.  Research studies have used ex vivo human blood, murine, and Rhesus macaque in vivo radiation models.  Here in, we focus to highlight our research capabilities and recent results as applied to prioritization of selection of suspected radiation casualties for cytogenetic – chromosome aberration dose assessment.  In addition, insights on a strategic approach to fill the critical biodosimetry gap will be discussed.  A description of our research program along with a list of relevant publications in these various sub-disciplines in biodosimetry are available at website.  [The views expressed here are those of the authors; no endorsement by the U.S. Department of Defense has been given or inferred.  Research was supported under AFRRI work units # RAB4AP and RBB4AR and DTRA Project #CBM.RAD.03.10.AR.002].